quantiFlash

APE exhibits at CYTO2018

April 28 – May 02 | Prague | Booth #1



Pulsed Calibration Light Source for Cytometers


Overview

quantiFlash® is an ideal tool to obtain the best resolution and sensitivity of cytometers. Unlike calibration beads, quantiFlash delivers consistent and uniform light pulses. Their intensity and duration is freely adjustable.

No Beads

Perfect light pulses with almost no variation outperform bead-based calibration particles. A precise LED pulser light source is the ideal tool for monitoring and calibration in flow cytometry.

Standard Protocols

Use standard calibration protocols or develop your own.

Performance Tracking & Result Comparison

Performance tracking in long-term or inter-laboratory studies as well as comparability of instruments become available with the quantiFlash LED pulser. quantiFlash enables user to determine the resolution and sensitivity (reflected by Q and B) for each detector so that instrument comparison as well as long-term experiments become more efficient.

Specifications

Specification quantiFlash 
Pulse Duration1.....10 µs
Other versions: up to 100 µs available
Repetition Rate500 Hz ..... 10 kHz | 100 Hz steps
Repetition Rate Precision10^-6
Pulse ShapeVariable (Gaussian as default)
Pulse Amplitude0 ..... - 96 dB
Amplitude Precision< 0.1 % CV
Triggeroptical and TTL
Fiber Coupling2 x fibers with f-SMA connector
Dimensions193 x 124 x 48 (L x W x H in mm)
PowerLi-ion battery (rechargeable)
USB powered
Options
Advanced PC software
Fixed attenuator
Customized fibers and connectors
Mounting Kit for BD Instruments

Contact Us






or

Email & Phone Contacts

APE Angewandte Physik & Elektronik GmbH
Plauener Strasse 163-165 | Haus N
13053 Berlin, Germany

tel +49 30 98601130
fax +49 30 986011333

Sales: sales@ape-berlin.de
Support: service@ape-berlin.de


quantiFlash Mounting Kit for BD available


quantiFlash Flow Cell Adapter for BD

quantiFlash comes with 2 fibers. The fiber ends have to be interfaced with the flow cytometer or to phrase it differently: 2 fibers = 2 points of connection of quantiFlash with the flow cytometer. For this our adapter can be easily mounted on BD instruments with access to the flow cell.

These are for example:

  • LSR II
  • FACSAria
  • FACSCanto
  • FACSCalibur
  • LSRFortessa

Cytometer Setup, Characterization and Standardization

Workshop 4: Sunday, April 29, 2018

quantiFlash | Beads | Cytometer setup | Q and B explained | Instrument operation point |  PMT & signal processing | Trending technical terms for cytometer characterization

Prague CYTO2018, Workshop 4

Building Measurement Assurance in Flow Cytometry

Workshop 13: Monday, April 30, 2018

Building measurement assurance in flow cytometry requires proper controls and standards, e.g. particles for instrument calibration, performance characterization, and standardization, biological cell reference materials, and validated measurement procedures. The workshop is intended to foster the information exchange between ISAC standards committee/task force, NIST, FDA, NIH, and user communities.

Prague CYTO2018, Workshop 13*

*This workshop is not associated with APE

Applications

Calibrate Intensity Scales to Absolute Units

• Ultra-stable light pulses allow for direct calibration of intensity channels to a standardized photonic scale
• Easy calculation of the corresponding number of detected statistical photoelectrons (Spe) for any intensity channel

Accurate Q and B Measurement

• Calculate values for detection efficiency (Q) and background (B) in terms of statistical photoelectron units (Spe) or fluorescence intensity units, such as mean equivalent soluble fluorescence units (MESF) or an equivalent number of reference fluorophores (ERF)
• Accurately compare different cytometers or setups

Performance Test of PMTs

• Use the proven perfect linearity of quantiFlash to check the performance of your PMTs and signal processing
• Precise adjustment of the delivered light intensity within up to 6 decades


Recommended Literature


One-page Overview: Photonic calibration, determination of background, signal-to-noise and dynamic range of a flow cytometer

Here we describe how quantiFlash is used to characterize a cytometer’s PMT performance and the instrument’s response over the entire PMT voltage range. The one-pager is available for download here.


Determination of background, signal-to-noise, and dynamic range of a flow cytometer: A novel practical method for instrument characterization and standardization

A well-defined scale calibration in flow cytometry can improve many aspects of data acquisition such as cytometer setup, instrument comparison and sample comparison. We employ a practical method to characterize a cytometer’s signal-to-noise ratio (SNR) and dynamic range (DNR). This allows the selection of a voltage/gain corresponding to a PMT’s maximum efficiency. The full paper is available at http://onlinelibrary.wiley.com/doi/10.1002/cyto.a.23250/pdf


Technical Note: Multispectral flow cytometry: The consequences of increased light collection

We use Q (detection efficiency) and B (background) values and develop a novel “multivariate population overlap factor” to characterize the cytometer performance. To verify the usefulness of our factor, we perform representative experiments and compare our overlap factor to Q and B. Finally, we conclude that the increased light collection of multispectral flow cytometry does indeed lead to increased sensitivity, an improved detection limit, and a higher resolution. The full technical note is available at http://onlinelibrary.wiley.com/doi/10.1002/cyto.a.22888/epdf


Forensic Flow Cytometry presented by NIH

Pratip Chattopadhyay, Staff Scientist at National Institutes of Health, presented a Forensic Flow Cytometry Tutorial at the CYTO2015 Conference. The full presenation is available at http://de.slideshare.net/PratipChattopadhyay/cyto-2015-forensic-flow-tutorial


Quantitative comparison study of flow cytometers using a novel ultra-stable calibration light source

In this talk we discuss ways to quantify flow cytometers in terms of sensitivity and resolution. As shown by others, the coefficient of variation (CV) of a stable light source can be used for scale calibration in numbers of detected photoelectrons. This calibration allows the quantitative comparison of flow cytometers in terms of light detection efficiency. The full presenation is available for download here: Calibration and Comparison Study with quantiFlash.


Comparison study of various flow cytometers presented by DRFZ

Comparison study of various flow cytometers using quantiFlash. Traditionally, flow cytometers are characterized (sensitivity, linearity, long time stability etc.) by fluorescent microspheres. As shown by others*) the coefficient of variation (CV) of a stable light source can be used for scale calibration in numbers of estimated photoelectrons. This allows the quantitative comparison of flow cytometers in terms of light detection efficiency. Due to the intrinsic CV (2-4%) of microspheres they are not suitable for such calibration. Here we show a comparison of the detection efficiency of 3 flow cytometers (FACSAria™) using quantiFlash™. quantiFlash™ is an ultra-stable (CV < 0.1%) easy to use LED pulse generator made for cytometer characterization. The poster has been presented at CYTO 2016, Seattle. This poster is available for download.


Isolation and characterization of Extracellular Vesicles presented by Beckman Coulter

Flow cytometric detection and sorting of EVs: analysis and characterization of background noise sources that may impact EV fluorescence or scatter detection by Dr. Carley D. Ross, Dr. Thomas Ramin, Dr. Aliaksandr Kachynski
Beckman Coulter Life Sciences,  Cellular and Molecular Life Sciences 2011; 68(16):2667-88. The full presentation is available at https://prezi.com/loogalklhahr/isolation-and-characterization-of-extracellular-vesicles/