Dual Color SRS with deltaEmerald

Dual Color SRS with deltaEmerald

Dual Color Stimulated Raman Scattering


Introduction

The new deltaEmerald allows simultaneous SRS imaging of two vibrational bands with its revolutionary dual color SRS (DC-SRS) scheme. It is aimed at users at the forefront of SRS microscopy, giving the best signal-to-noise performance and the capability of background subtraction as well as simultaneous imaging of two vibrational bands.

Two Stokes pulses, separated by 85 cm-1 and modulated at different frequencies are overlapped with the tunable Pump pulse.

The pulse length of about 1 ps, 10 cm-1 … 15 cm-1 bandwidth and several 100 mW output power in each beam are ideal parameters for Coherent Raman imaging.

The pulses of all three beams are shot noise limited due to solid state laser design and proven OPO technology. The shot noise limit of -162 dBc/Hz above 1 MHz (limited by the noise measurement setup with 4.8 mA) allows for fast image acquisition and superior signal-to-noise ratio.

Fully automated tuning, power control and temporal and spatial overlap of all three beams are given.

Additionally a ~100 fs output at 1030 nm is provided for efficient SHG and two-photon imaging.

 

For more details regarding Coherent Raman Microscopy, please look here.


Virtual Histology (Colon)
Diverse applications

At a Glance

  • Innovative SRS scheme for simultaneous excitation of two vibrational bands
  • Tunable Pump and both Stokes pulses are spatially and temporally overlapped
  • Spectral distance of Stokes beams 85 cm-1
  • Proven OPO technology with shot noise limited performance with -162 dBc/Hz above 1 MHz
  • Additional femtosecond output at 1030 nm beam for optimum SHG and TPF excitation

Applications

  • Simultaneous imaging of CH2 & CH3 for nondestructive and fast Coherent Raman histology. The 85 cm-1 energy difference is chosen to address CH2 & CH3 with 2845 cm-1 and 2930 cm-1 at their peak position.
  • Background removal of non-resonant artefacts, especially important for weak signals in the fingerprint region
  • Cream spreading quantification, Drug penetration, Metabolic imaging, Microplastics discrimination, Plant imaging, Cell imaging, Fingerprint imaging, etc.

Specifications

Applications

deltaEmerald
Wavelength 1 (Stokes 1)1034 nm
Wavelength 2 (Stokes 2)1025 nm
Wavelength 3 (Pump - Signal)740 nm … 990 nm
∆v Pump - Stokes350 cm-1 … 3800 cm-1
Power Stokes 1>300 mW (unmodulated), >150 mW modulated (20 MHz)
Power Stokes 2>300 mW (unmodulated), >150 mW modulated (13 MHz)
Power Pump (Signal)>400 mW at 800 nm
Spectral bandwidth10 cm-1 … 15 cm-1 (Pump and Stokes)
Pulse width1 ps
Repetition rate80 MHz
NoiseShot noise limited (-162 dBc/Hz) >1 MHz for Pump and Stokes
Beam parameters
Pointing stability<100 µrad per 100 nm
<1.2 for Pump and Stokes
Ellipticity<20%
PolarizationLinear, horizontal >100 : 1
Beam divergence0.9 mrad ± 0.2 mrad at 800 nm and Stokes
Beam waist diameter1.3 mm ± 0.2 mm at 800 nm, 1.7 mm ± 0.2 mm for Stokes
Additional femtosecond output
Wavelength1030 nm ± 3 nm
Power300 mW
Pulse width<200 fs
Beam divergence<1.5 mrad
Features
Integrated modulator for StokesBuilt-in EOMs with resonant fixed frequency of 20 MHz and 13 MHz
Power attenuatorsIntegrated for Pump and Stokes, independent for each beam
Delay managementAutomated compensation for microscope dispersion
ConnectivityEthernet and Software interface TCP/IP
  • Simultaneous imaging of CH2 & CH3 for nondestructive and fast Coherent Raman histology. The 85 cm-1 energy difference is chosen to address CH2 & CH3 with 2845 cm-1 and 2930 cm-1 at their peak position.
  • Background removal of non-resonant artefacts, especially important for weak signals in the fingerprint region
  • Cream spreading quantification, Drug penetration, Metabolic imaging, Microplastics discrimination, Plant imaging, Cell imaging, Fingerprint imaging, etc.

Datasheets & Brochures

APE deltaEmerald rev.1.0.0 (pdf / english)


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Dual Color Stimulated Raman Scattering with deltaEmerald full compatible with DC-CRS microscope by Lightcore Technologies.