The new Chameleon compact OPO (Optical Parametric Oscillator) is the first completely hands-free, fully automated and computer controlled OPO, which - as specific feature - is fully flexible in pump- and OPO output wavelengths at the same time. This allows the use of any wavelengths combinations.

In combination with the Chameleon pump laser from coherent it is the perfect excitation source for MPE (Multiphoton Excitation) microscopy with a wavelength range of 680…1600 nm, expandable with a SHG/THG module down to UV (<230 nm) without any gap.

Significant advantages for laser scanning microscopy are:

- a higher penetration depth and lower sample photo damage due to excitation wavelengths >1 µm,

- the possibility of simultaneous imaging of different fluorophores, for instance exciting GFP (green fluorescent protein) with the laser and RFP (red fluorescent protein) with the OPO,

- the multimodality of the system, which gains access to alternative imaging methods like coherent Raman (CARS/SRS) and SHG/THG in addition to MPE.

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